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Home Biotechnologies Mikrotytrator for Serological Tests and Scientific Studies Connected to Direct and Indirect Imunoflyuorestsent the Method of Hanging Drops

Mikrotytrator for Serological Tests and Scientific Studies Connected to Direct and Indirect Imunoflyuorestsent the Method of Hanging Drops

Description

The objective is to create “Mikrotytrator” which provides mikrotytration in serial dilution on a flat work surface, such as substantive glass formicroscopy needed for studying thematerial using the methods of direct and indirect imunoflyuorestsently and hanging drop method, and so on.

The technical nature provides itswork, so “Mikrotytrator” works as follows: by feeder 1 with the nipple 2 through the outlet 3 it is filled with solvent in volume equal to the volume of fluid capillary vessels 4. Thus, the studied capillary vessel 4 filled with fluid is overflown and ismaintained bymechanism5 in the start point opposite the nozzle 3 pipe 2. Due to the overflow of capillary vessels the investigated liquid mixes with the solvent so the first fluid dilution is obtained. It titrates with the Score titer of 1:2. Thus one half of the received mixture fills the cavity of the capillary vessels and is equal to its volume, and the second half of themixture outside covers (envelops) capillary vessels and is held by the surface tension of liquid. With the help of mechanism5 overflown capillary vessel approaches a flatwork surface 7 at a sufficient distance to ensure the transfer to work surface 7 the part of liquid that wets the certain area of the work surface of substantial glass 8 and remains on it as a drop, detached from the capillary vessels . This gap occurs due to the separation of capillary vessels fromthe work surface at the backwardmove of the capillary vessels bymechanism5 in the start point, that is installing of the capillary vessels 4 in front of the nozzle 3 pipe 2 feeder 1. Then, using the mechanism 10 the objective table 9 with the slide 8 is moved to a horizontal surface at a given distance - and thus provides a free work surface for the application of a new dilution . Subsequent liquid dilutions with the increasing titer score according to a geometric progression of 1:4, 1:8, 1:16 etc. are applied by successive repetition of the cycle of these operations to the new free sites of a work surface and thus the mikrotytration is obtained.

Innovative Aspect and Main Advantages

According to the description of the technicalmerits device and the beforementioned research, we proved the possibility of mikrotytration on a flat surface, which gives grounds to consider this mikrotytrator as a useful model.

This utility models differs from existing analogue in a way that it allows to obtain mikrotytration on a flat work surface, such as substantive glass for microscopy.

Areas of Application

  • Pharmacology;
  • Immunology;
  • Medicine;
  • Microbiology;
  • Veterinary medicine;
  • Biology;
  • Protection of animals;
  • Virology;
  • Chemical.


Stage of Development

  • Tested, available for demonstration – field tested;
  • Patented.

Contact Details

Lviv Research Institute of Epidemiology and Hygiene Ministry of Health of Ukraine
Contact person: Director, PhD Vsyaka Iryna
Address: 12, Zelena str., 79005, Lviv, Ukraine
Тel.: (+38 032) 276 2832
Fax: (+38 032) 276 3067
E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it
E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

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