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Home Biotechnologies A Novel Microfluidic Analytical System For Electrochemiluminescent Determination And Capillary Electrophoresis Separation Of Biologi-Cal Molecules In Bioliquids (Blood, Blood Plasma, Urine)

A Novel Microfluidic Analytical System For Electrochemiluminescent Determination And Capillary Electrophoresis Separation Of Biologi-Cal Molecules In Bioliquids (Blood, Blood Plasma, Urine)


In this work, microfabrication technology has employed to develop a novel microfluidic analytical system for electrochemiluminescent (ECL) determination and capillary electrophoresis (CE) separation of biological molecules in bioliquids (blood, blood plasma, urine). This system can be used for biomedical applications like diagnostics.

Microfluidic platform with micron-sized channels (U-turn separation microchannel and double-T nanoprobes injection layouts) were fabricated using a photosensitive glass submerged etching technique. By means of Langmuir-Blodgett (LB) technology ECL reactant has been permanently immobilized on an electrode surface of microfluidic chip. Amino acids have been selected as test components for probing of the ECL–CE microfluidic system.

Innovative Aspect and Main Advantages

Innovative Aspect

  • Combination of ECL and CE techniques in one microfluidic analytical system provides gaining in selectivity and sensitivity of assay’s protocol.
  • Facilitation of ECL detection by immobilization of ECL reactant on the electrode surface by LB technique.
  • Using CE as electrokinetic pumping alternative to pressure-based pumping.

Main advantages

  • Detection limit. 10-15M for ECL method in comparison with 10-7M — for electrochemical and 10-9 M — for fluorescent methods.
  • Selectivity. Highly efficient biochemical CE separation allow to increase selectivity of ECL detection in comparison with ECL method alone. Other separation methods such as high-performance liquid chromatography (HPLC) also have high separation power, however it is difficult to adapt in microformat because of microliters injection volume compared to nanoliters for CE.
  • Reactant saving. Infinitesimal of ECL reactant by it’s permanently immobilization on the electrode compared to great enough amount of ECL reactants used in solution.
  • Buffer consumption or channel volume. 2 μl compared to 2ml for similar macroscale systems.
  • Simplification of sensor structure. By CE pumping and by permanently immobilization of ECL reactant on an electrode, not require addition instruments for sample and reactant delivery compared to pressure-based pumping and using ECL reactants in solution.
  • Working area. Less then 6 cm2 compared to 50 cm2 for similar macroscale systems.
  • Minimization of the detection volume. Nanoliters sample volume (0.25 nl) for CE compared to microliters for flow injection analysis or HPLC.

Additional advantages

  • Potential for batch fabrication.
  • Cheep production.
  • Reducing of power consumption.

Possibility for integrated electronics, signal processing and detection.

Areas of Application

  • Neurochemistry: serotonin, dopamine, adrenaline.
  • Enzyme/immunoassays: histamine, insulin, glucose.
  • Clinical diagnostics: uric acid, amino acids.
  • Environmental assays: nitroaromatics, organophosphates.

Fig. 1 Schematic and photograph of microfluidic platform design:
a — sketch of the microfluidic platform (Reservoirs: 1 –buffer
solution, 2 – sample, 3 – sample injection waste, 4 – waste, and
5 — separation U-turn microchannel)
b — photograph of microfluidic platform.
c — double-T injection channel (enlargement of the photograph)

Stage of Development

Development phase — laboratory testing of microfluidic platform

Contact Details

Prof. Mykola Rozhitskii, Katerina Muzyka
Kharkiv National University of Radioelectronics, Biomedical Elecrotronics Chair, Lab. of Analytical Optochemotronics
Address: 14 Lenin Ave., Kharkiv, 61160, Ukraine
Phone: +38 057 7020369, Fax: +38 057 7021013
E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

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