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Home Biotechnologies ECL Sensor For Assay Of Bioliquids

ECL Sensor For Assay Of Bioliquids

Description

The essence of our proposal is sensor aimed at assay of liquid mediums (in particular bioliquids) in medicine and environmental monitoring. It is based on combination of two main ideas: electrochemiluminescent (ECL) detection method and electrodes coated with condensed thin films doped with electrochemiluminescers reactants.

ECL assay method possesses a) high sensitivity due to optical detection of signal that nowadays allows reaching single-photon detection limit; b) high selectivity of the assay due to two-channel registration mode, i.e. simultaneous recording of electrochemical and optical ECL signals.

Incorporation of electrochemiluminescer – reactant into film deposited onto electrode allows us to use those reactants in mediums where they are insoluble/unstable and thus broaden the range of analytes that can be detected and mediums where the assay can be done. The method that is used for thin films formation that are further deposited onto working electrodes of the proposed sensor is Langmuir-Blodgett. It allows formation of uniform ordered monomolecular films with highly controllable predefined structure.

The proposed sensor is aimed at detection of certain aminoacids in bioliquids (urine and blood) that is important for early recognition of metabolic disorder and diseases accompanied by this disorder (e.g. triptophanurea). Further broadening of analytes and liquid mediums range for the assay is possible on the base of the described concept.

Innovative Aspect and Main Advantages

Innovative aspect of the proposition consists in combining analytical capabilities of liquid-phase electrochemilumines-cence and the Langmuir-Blodgett technology for electro-chemiluminescers thin ordered layers formation and deposition onto sensors’ electrodes. This will make it possible to utilize electrochemiluminescers, which could not be used in bioliquids before as reactants and thus broaden the range of analytes that can be detected.

The detection of certain aminoacids is possible due creation of new ECL composition/ sensor structure that are capable of working in aqueous systems (a matter of patent application). Main advantages of the developed sensor are:

  • low limit of detection (down to 10-8 mole/l);
  • fast and simple assay procedure and sample preparation (approx. 1 - 2 min., requires only one person);
  • low sample volume (~20 μl).

Areas of Application

Assay of bioliquids (urine and blood) for aminoacids content (that is important for early recognition of metabolic disorder and diseases accompanied by this disorder). Assay of other mediums is under development.


Fig. 1 ECL sensor cell with accessory for experiments
(without external quartz cell)

a) b) Fig.2 Analytical voltage-current and voltage-photocurrent responses of ECL sensor using method of cyclic voltammetry for model system a) containing no analyte (optical response about 100 pA) b) containing analyte (optical response about 50 nA, i.e. 2.5 orders of magnitude higher).

Stage of Development

Development phase – laboratory tested.

Contact Details

Dr. Sci. Phys.- Math., Prof. Mykola M. Rozhitskii
Kharkiv National University of Radio Electronics
Ukraine, 14 Lenin Ave., UKR-61166 Kharkiv,
TEL: +38057-7021413, +38057-7020369,
FAX: +38057-7021013.
E-Mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it , This e-mail address is being protected from spambots. You need JavaScript enabled to view it

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