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Home Biotechnologies Evaluation Of The Effectiveness Of Osteoprotectors In Osteoporosis According To Cytomorphological Indices

Evaluation Of The Effectiveness Of Osteoprotectors In Osteoporosis According To Cytomorphological Indices

Description

Osteoporosis is a widely-spread disease stipulated by a sedentary life (hypokinesia), genetic factors, unfavourable ecological conditions and is considered as the "disease of civilization". Mortality from consequences of osteoporosis (fractures, insults etc.) is high enough, particularly in elderly people. Elucidation of mechanisms of osteoporosis development and elaboration of effective protectors appear to be rather actual. Many-year investigations conducted by us during studies of cytological peculiarities of osteoporotic development under microgravity and hypokinesia conditions allowed us to work out methodical approaches and criteria for recognizing osteoporosis and osteopenia.

To elucidate the effectiveness of osteoprotector preparations and technologies, we propose the following evaluation indices of bone tissue state which are applied in standard histological sections obtained from bone structure biosamples and biopsies: osteocyte indices (iOS 1 and iOS 2); osteoblast – osteoclast indices (iOB/OC); index of the blood – vascular bed state (iBVB); bone tissue "porosity" index (iBP); mineralization index (iM) and oth.

Also informative are the following methodical aproaches: colony formation intensity and differentiations of osteogenous precursor-cells under ex vivo conditions; evaluation of the intensity of osteogenetic and resorptive processes according to the functional state of bone tissue cells. Our findings testify to a reliability of these indices for identification of osteoporotic changes in bone tissue. Positive results have been obtained showing informativity of these indices when evaluating the effectiveness of osteoprotectors we developed. They were created on the basis of low-molecular metabolites of natural origin (osteoprotector NV and osteoprotector NT), and were forwarded for treating osteoporosis (Fig.1). The investigations are being conducted with the use of the computer programmes for image analysis in the light microscope.

Innovative Aspect and Main Advantages

Original methodical approaches (indices) have been developed for identification and analysis of osteoporotic rebuildings in bone tissue which are necessary for standardization in evaluating the effectiveness of osteoprotectors during their elaboration (pharmacological preparations of bio-additions and various bio-medical technologies).

Main advantages of the proposed methodical approaches are:

  • the possibility of use of indices for ascertaining the availability of osteoporotic changes in bone tissue; relative simplicity of use
  • correct (proper) comparison of the experimental results: normal state of bone tissue – osteoporotic changes of various aetiology – effects after application of osteoprotectors
  • informativity of the proposed indices of bone tissue state has been confirmed in our experiments with modeled hypokinesia which provokes development of osteopenia and osteoporosis.
  • the proposed osteoprotectors are not hormonal preparations, highly-effective in relation to bone tissue mineralization.

Areas of Application

The proposed approaches can be applied in medicine, pharmacology and biology for evaluation of the effectiveness of osteoprotectors.


Fig. 1 Fragments of bone tissue histological sections
a – changes in osteocytes in osteoporosis;
b – after application of the osteoprotector NV;
A change of the osteocytic index is seen on the ordinate axis.

Stage of Development

With the application of the developed methods the experimental work is being carried out on the creation of osteoprotectors NV and NT. Preclinical tests are being conducted on laboratory animals.

Contact Details

Schmalhausen Institute of NAS of Ukraine
Rodionova Natalia Vasilievna
Address: B. Khmelnitsky st.,15, Kiev-30, MSP, 01601 Ukraine
Tel/Fax: +380 44 2349084
E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

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