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Home Georgian Technologies Design of Capillary Electrophoresis Device With an Absorption Detector

Design of Capillary Electrophoresis Device With an Absorption Detector

Description

In the existing absorption detectors, despite the intensity of light stabilization, the output signal is continuously affected by the changes in the intensity. This interference the absorption signals which are comparable to or less than the signals resulted from the modulations in the intensity of light. This phenomenon limits the detection threshold. The output signal also is affected by instabilities in the coefficients of the detector amplification and of the amplifier that also limits the detection threshold. The coefficient of amplification may change due to instability of applied voltage or changes in temperature. A new absorption detector, which is to be constructed for the proposed CE device, will enable us to transmit the light beam periodically through the absorption area of a capillary. During the first period the beam passes through the area of the capillary where there is no solution fraction (which is to be analyzed). The obtained electric signal (reference signal) is transmitted to the so-called reference channel. During the second period, the beam passes through the area of the capillary where there is a fraction which is to be analyzed. The obtained electric signal (basic signal) will be transmitted to the so-called basic channel. Then the reference signal is detracted from the basic one and the obtained difference is measured.

Innovative Aspect and Main Advantages

In comparison with the existing ones, the proposed absorption detector has the following advantages:

  • The output signal is affected only by a small part of the noise from different units of the device that increases the signal/ noise ratio and improves the detection threshold;
  • The changes in the electric signal, which are caused by the changes of light source intensity, are effectively compensated.
  • The ratio signal/noise increases and the detection threshold is improving. The changes in the output signal, which are caused by instabilities of the coefficients of amplification of the photo-detector and the amplifier, are also effectively compensated, that also improves the detection threshold;
  • The proposed system allows to measure the absorption signal in units of optical densities that simplifies the connection of measured signals to the concentrations of fractions under investigation;
  • The design of the detector enables us to use a unique scheme of measuring light absorption. This scheme makes possible to measure much weaker absorption signals that significantly improves the detection threshold of the method.

Thus, the use of the proposed CE device sharply improves the detection threshold and the accuracy of the method.

Areas of Application

This device can be used in analytic laboratories in fundamental as well as in applied research. It can be used in medicine (for example for the early diagnostics), biology, chemistry, physics, ecology, biotechnology, pharmaceutics, agricultural science, food processing industry. All this shows that the proposed project has great scientific value.

The developed design will enable us to build a low-cost, simple, high-sensitivity device, which can be produced in series. That means that the project is interesting also from commercial point of view.


 

 

 

 

 

 

 

Fig.1. Block diagram of the device.
1- Light Source; 2. Monochromator;
3. Light beam operation;
4. Capillary; 5. Photo –detector;
6. Amplifier; 7. Registration block.

Stage of Development

Conceptual stage.

Contact Details

Andronikashvili Institute of Physics
6 Tamarashvili St.0177 Tbilisi, Georgia
Contact person: Dr. Alexsander Rcheulishvili
Tel: (+ 995 32) 39 8783
Fax: (+995 32) 39 1494
E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it , This e-mail address is being protected from spambots. You need JavaScript enabled to view it

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