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Home Material Sciences Electrochemiluminescent sensor for assay of bioliquids

Electrochemiluminescent sensor for assay of bioliquids

Description

The essence of our proposal is sensor aimed at assay of liquid mediums (in particular bioliquids) in medicine and environmental monitoring. It is based on combination of two main ideas: electrochemiluminescent (ECL) detection method and sensor which electrodes inner surfaces are prepared on a base of nanotechnology approach by coating with ordered molecular thickness films doped with electrochemiluminescers reactants. ECL assay method possesses:
i) high sensitivity due to optical detection of analytical signal that allows reaching single-photon detection limit;
ii) high selectivity of the assay due to two-channel registration mode, i.e. simultaneous recording of electrochemical and optical (ECL) signals.

Incorporation of electrochemiluminescer – reactant into film deposited onto electrode allows using those reactants in mediums where they are insoluble/unstable and thus broadening the list of analytes that can be detected and mediums where the assay can be done. The nanotechnology approach that is used for thin films formation and their further deposition onto working electrodes of the proposed ECL sensor is Langmuir-Blodgett method. It allows formation of uniform ordered monomolecular films with highly controllable predefined structure.The proposed sensor is aimed at detection of certain aminoacids in bioliquids (urine and blood) that is important for early recognition of metabolic disorder and diseases accompanied by this disorder (e.g. tryptophanurea).

Innovative Aspect and Main Advantages

Innovative aspect of the proposed technology consists in combining analytical capabilities of liquid-phase electrochemiluminescence and the Langmuir-Blodgett technology for electrochemiluminescers thin ordered layers formation and deposition onto sensors' electrodes. This makes it possible to utilize electrochemiluminescers as reactants, which could not be used in bioliquids before, thus broadening the range of detected analytes important for biomedical and ecological applications.The detection of certain aminoacids is possible due creation of new ECL composition/ sensor structure that are capable of working in aqueous systems.

Main advantages of the developed sensor are:

  • low limit of detection (up to 2.5 x 10-7 M);
  • fast and simple assay protocols and sample preparation (approx. 1–2 min., requires only one person);
  • low sample volume (~20 μl);
  • wide linear detection range 10-7 - 5 x 10-3 M;
  • fully automated assay procedure.

It is competitive with known analytical technologies for bioliquids assay such as fluorescent, electrochemical etc.

Areas of Application

The developed sensor can be used for ECL assay of aqueous mediums including bioliquids. In particular it can be applied in:

  • clinical assay;
  • biological assay;
  • environmental assay.

Other application areas can include pharmaceutical and food products.

ECL sensor module with accessories for assay accomplishment
Fig. 1 ECL sensor module with accessories for assay
accomplishment


Fig. 2 Calibration curve of ECL sensor with model analytical system.
Linear detection range 10%7 % 5?10%3 M,
limit of detection 2.5x10-7 M (S/N=3)

Stage of Development

Development phase – laboratory tested samples and assay protocols are available.

Contact Details

Contact persons: Dr. Sci., Prof. Mykola Rozhitskii, Jun. Res. Yuriy Zholudov.
Lab. of Analytical Optochemotronics, Dept of Biomedical Electronics, Kharkiv National University of Radio Electronics,
14, Lenin Ave., Kharkiv, 61160, Ukraine
Phone: +38 057 7020369
Fax: +38 057 7021013
E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

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