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Home Moldova Technologies Virus Free Grapevine Plantlets Production

Virus Free Grapevine Plantlets Production


The International Council for the Study of Virus and Viruslike Diseases of Grapevine recognizes over 70 infectious agents affecting this culture. Many of them cause disorders that reduce the plant vigour and longevity, the quality and quantity of the yield. Infected propagating material is largely responsible for the spread of these diseases among and within viticulture regions. According to existing legislation all vineyards must be founded using only virus free material.

The meristem tip culture is used for elimination of harmful viruses. Grapevine shoot tips are micropropagated on different variants of Murashige & Skoog medium selected for each genotype.

In order to obtain virus free plants an individual protocol for virus eradication is necessary (only meristem culture, meristem culture and thermotherapy or chemotherapy). For confirmation of virus elimination the plantlets were tested out in virus detection. Virus free plantlets may by used for mass micropropagation and foundation of a depository of virus free stock plants.

Innovative Aspect and Main Advantages

Meristeme culture is a unique technique to produce plants free from various pathogens including viruses, viroides, mycoplasma. Benefit of using meristem culture as a means of regeneration consist in production of stabile forms and virus free stock plants.

Meristem derived plantlets carry many quantitative and qualitative performance. The productivity of healthy plants is higher; the resistance to unfavourable climatic conditions increase; the fruit yield quality of meristem derived plants is higher than of runner derived plants.

Areas of Application

Virus free depository-collections are subject of all Grapevines genetic resources, including valuable genotypes with resistance to unfavourable biotic and abiotic factors. The increasing security for grapevine genotypes has been asked by Agricultural Department, Scientific Institutes of the Academy of Sciences, private organizations


Elimination of the harmful viruses by meristem culture

Stage of Development

  • Technical protocols of standard plantlets production are developed.
  • Pilot lots of local cultivars are obtained.
  • Developed algorithms are available for other horticulture species.

Targeted Companies:

On virus free plantlets are interested:

  • big and middle producers;
  • producers from other viticulture countries interested in our new cultivars resistant to unfavourable factors;
  • breeders and viticulture centres interested in autochthonous varieties as source of high quality and productivity.

Contact Details

Institute of Genetics and Plant Physiology
Contact person: Larisa Andronic, PhD, Deputy director
Address: 22 Padurii St. Chisinau 2002, Republic of Moldova
Tel: (373 22) 66 04 09
Fax: (373 22) 55 61 80
E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

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