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Home Sensors Surface plasmon resonance spectrometer plasmon-5 for biosensing

Surface plasmon resonance spectrometer

PLASMON-5 for biosensing

Description

Surface plasmon resonance (SPR) is a unique optical surface sensing technique that is responsive to refractive index changes that occur within the vicinity of a sensor surface. Thus, SPR can be used to monitor any physical phenomenon which alters the refractive index at the surface and has grown into a versatile technique used in variety of applications. Of special interest is its potential for biosensing techniques

Innovative Aspect and Main Advantages

Biosensor applications of SPR spectrometer rely upon high selectivity of biomolecular interactions. In typical biosensor, the biosystem sample is placed onto derivatized (usually with alkyl thiols) gold film using appropriate immobilization techniques (covalent, His or affinity tag, antibodies). Selective coupling of the analyte species present in the probe to the immobilized biosystem affects the effective refractive index in the vicinity of the biosensor surface resulting in a resonance curve shift. An advantage of SPR method in comparison with other biochemical methods is absence of molecular labeling. Depend on modification device has one or two optical channels. Second channel can be used as measuring or reference. Modification of the instrument destined for electrochemical applications features additional ADC input and software means for recording of voltamperograms simultaneously with the optical signal.

Areas of Application

Immunoassay and biosensing techniques (veterinary, foods industry, environmental monitoring), monitoring of biochemical kinetics, refractive index measurements and thin film characterization, in situ electrochemical studies, bulk liquid measurements, educational activities.

Surface plasmon resonance spectrometer PLASMON-5
Fig. 1 Surface plasmon resonance spectrometer PLASMON-5

Stage of Development

Prototype developed and tested.

Contact Details

Contact persons: Yu.V. Ushenin, A.V. Samoylov
V. Lashkaryov Institute of Semiconductors Physics of the National Academy of Sciences of Ukraine
Address: 41, Prospect Nauki, 03028, Kiev, Ukraine
(+38 044) 525 3123, 525 5743
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