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Home Uzbekistan Technologies Novel Methods to Produce Cotton Somatic Embryoids from Gossypium Hirsutum L. and Other Cotton Species

Novel Methods to Produce Cotton Somatic Embryoids from Gossypium Hirsutum L. and Other Cotton Species

Description

Intensive development of methods for cultivation of isolated plant organs, tissues and cells is widely applied not only as a product of secondary metabolism but as a model system for study of differential gene expression and material for plant genetic manipulation. For plant regeneration in vitro a lot of researchers cultivated successfully hypocotyl, leaf, stem, apex and other parts of plants. Glandular trichomes obtained from 6 days cotton plantlets were used as explants in the Institute of Genetics and Plant Experimental Biology AS RU. The glandular trichomes of 13 cotton genotypes and their hybrids were studied under different water-stress conditions. Genotypes with the largest number of original material have been isolated and methods of obtaining and cultivation from cotton glandular trichomes has been developed.

Innovative Aspect and Main Advantages

Glandular trichomes of cotton, which can be obtained during 14-21 days from sterile 6 days plantlets and used in tissue culture for different biotechnological investigations, were utilized as explants for the first time.

Areas of Application

Glandular trichomes can be used as explants in vitro conditions and for identification of chemical agents and molecular- genetic investigations.


Fig. 1 Somatic embryoids of G.hirsutum L. (x 16).

Stage of Development

Methods for obtaining of callus tissue from cotton glandular trichomes, callus formation and embryogenesis have been developed. Laboratory tested.

Contact Details

Institute of Genetics and Plant Experimental Biology
Contact person: Academician M. Akhmedov
Address: Yukori-Yuz, 102007 Tashkent region
Tel: (+998 71) 264 7867
Fax: (+998 71) 264 2230
E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

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